Protocols

Protocols for Macromolecule Quantification

• Carbohydrate
  • Total particulate carbohydrate from microalgae (YY Hu, ZV Finkel, 2022) protocols.io, 10.17504/protocols.io.yxmvmk24ng3p/v1
• Lipid
  • Rapid extraction of total lipids from microalgae (YY Hu, ZV Finkel, 2022) protocols.io 10.17504/protocols.io.dm6gpr9jdvzp/v5
  • Lipids in microalgae: Quantitation by acid-dichromate method in microtiter plate (YY Hu, ZV Finkel, 2022) protocols.io 10.17504/protocols.io.e6nvw9dpzgmk/v2
  • Estimate phospholipids from microalgae (YY Hu, ZV Finkel) protocols.io 10.17504/protocols.io.q26g74r19gwz
• Protein
  • Chloroform-methanol protein precipitation from microalgae and Pierce BCA assay (YY Hu, C Lord, ZV Finkel, 2022) protocols.io, 10.17504/protocols.io.yxmvm2e25g3p/v1
• Nucleic acids
  • Total RNA and DNA in microalgae (YY Hu, ZV Finkel, 2022) protocols.io 10.17504/protocols.io.6qpvro85bvmk/v11
• Phosphate
  • An X-HTDC method for estimating particulate phosphorus from microalgae (YY Hu, ZF Finkel, 2022) protocols.io. 10.17504/protocols.io.kqdg35dq7v25/v6
  • DAPI-Based Polyphosphate Estimation with Extraction Sufficiency Validation: A Method for Quantifying Polyphosphate from Microalgae Samples (YY Hu, ZV Finkel, 2023) protocols.io 10.17504/protocols.io.ewov1ox87lr2/v1
  • Inorganic polyphosphate in microalgae: A DAPI-based quantification in microtiter plate (YY Hu, ZV Finkel, 2022) protocols.io
• Silicate
  • Measurement of biogenic silica from phytoplankton (YY Hu, N Samarasinghe, ZV Finkel, 2023) protocols.io 10.17504/protocols.io.8epv5jjzjl1b/v2
• Related protocols
  • Preparation of oxalate reagent (YY Hu, ZV Finkel, 2021) protocols.io 10.17504/protocols.io.dm6gpr6yjvzp/v2

Datasets

Stoichiometry

• RM Sheward, JD Liefer, AJ Irwin, ZV Finkel (2023) The Emiliania hyxleyi stoichiometry database. 10.5281/zenodo.7594880

Effects of oil on phytoplankton

• Eukaryotic community response to oil and Corexit in a coastal water mesocosm, (Mesocosm 1, August 2015.) Eukaryote RNA sequencing on GRIIDC UDI R4.x263.189:0010 and doi 10.7266/n7-kvpb-0878
• Eukaryotic community response to oil and Corexit in a mesocosm, (COAST, Mesocosm 2, October 2015.) Eukaryote RNA sequencing on GRIIDC UDI R4.x263.189:0011 and doi 10.7266/n7-43q9-xq06
• Metatranscriptome analysis of COAST mesocosm on GRIIDC UDI R6.x807.000:0072 and doi 10.7266/2JWNDY5F
• Eukaryotic phytoplankton community composition in the COAST (Gulf of Mexico, COASTal water mesocosms amended with coastal microbial concentrate) mesocosm as identified by 18S sequence from RNA-seq whole transcriptome sequencing for four treatments (control, WAF, CEWAF, dilute CEWAF). 18S rRNA sequences for Mesocosm 2 data on GRIIDC UDI R6.x807.000:0040 and doi 10.7266/032DBZJY
• Sensitivity index for phytoplankton on figshare 10.6084/m9.figshare.8766389.v1
• Percent relative abundance of the eukaryotic microbial community in the GOMCOAST (Gulf of Mexico, Coastal Water, mesocosm 4) mesocosm. Eukaryote RNA sequencing for Mesocosm 4 on GRIIDC UDI R4.x263.000:0042 doi 10.7266/N7JD4V8Z
• Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments. Results from exposure experiments with six different microalgae species. Data on GRIIDC UDI R6.x807.000:0014 doi 10.7266/n7-jehv-5771
• Response of toxigenic Pseudo-nitzschia sp. to oil and dispersant exposure over 6 days. Results from exposure experiment on Pseudo-nitzschia sp. Data on GRIIDC UDI R6.x807.000:0035 doi 10.7266/n7-qpqz-hn88
• NCBI BioProject PRJNA489497.

Chaetocerous affinis transcriptome

• NCBI BioProject PRJNA495610.

Macromolecules

• Macroalgae data on Figshare at doi 10.6084/m9.figshare.4248962
• Phytoplankton macromolecular composition and cell size data on figshare at 10.6084/m9.figshare.1600986.
• N starvation effects on macromolecular content for four phytoplankton 10.1371/journal.pone.0195705.t001, 10.1371/journal.pone.0195705.t003

Evolution

• Micromonas polaris evolution data on Figshare at doi 10.6084/m9.figshare.4264499

Niches

• Diatoms and dinoflagellates in the North Atlantic from CPR on Pangaea 10.1594/PANGAEA.778968